Postdoctoral position: Rules for efficient production of proteins with posttranslational modifications
Lund, Skåne
Tillfälligt
Heltid
16 timmar sedan
Offer DescriptionThe lab of Kristine Steen Jensen is looking for a highly motivated and self-driven postdoctoral research-candidate for the development of systems for producing proteins with posttranslational modifications (PTMs) through expansion of the genetic code. The project aims to develop systems for production of posttranslational modified proteins through recombinant expression and incorporation of non-canonical amino acids. The focus of the project is to identify key determinants for high-yield expression, coupling codon usage and primary and secondary structure to translation levels upon incorporation of non-canonical amino acids.Job description:The project has a biochemical focus, and the candidate is expected to develop a high throughput assay to study the coupling between local codon usage and expression yields of proteins with noncanonical amino acids. The work will include generation of DNA libraries, development of cell-free expression systems for screening of incorporation of noncanonical amino acids, fluorescent based quantification of expression levels, and development of data analysis strategies for the generated high throughput data. The proteins produced in this project will then be used to investigate the influence of PTMs on structure, function and dynamics using biophysical methods.Where?The researcher will join KS Jensen research group that studies the biophysical characterizations of the impact of posttranslational modifications on protein structure, function and dynamics. We are part of Division for Biophysical Chemistry, Center for Molecular Protein Science ( ), Department of Chemistry, Lund University, Sweden. Ongoing research at the Division of Biophysical Chemistry includes studies of biomolecular interactions, structure, and dynamics, aiming to understand biological systems and processes at the molecular level. The focus is on experimental and theoretical studies of the physical properties of proteins in solution. We have state of the art facilities for protein chemistry and protein NMR spectroscopy.Qualifications:The candidate should hold a PhD or equivalent degree in a relevant field such as biochemistry or molecular biology. The candidate should be self-motivated and independent, be collaborative, and be interested in contributing to the common good of the lab. Good skills in oral and written English are required.The candidate must have experience in molecular studies applied to biological macromolecules. Previous experience with flow cytometry or FACS is beneficial. An interest in automation of experimental work using robotic setups is also beneficial.How to apply:Send an email to with your CV (max. 2 pages) and a cover letter stating your interest and background (1 page).Where to apply E-mailkristine.steen_jensen@bpc.lu.seRequirementsResearch Field Chemistry » Biochemistry Education Level PhD or equivalentResearch Field Biological sciences » Biological engineering Education Level PhD or equivalentAdditional InformationWork Location(s)Number of offers available 1 Company/Institute Lund University Country Sweden City Lund GeofieldContact CityLund WebsiteStreetPO Box 124 Postal CodeSE-22100 E-Mailkristine.steen_jensen@bpc.lu.seSTATUS: EXPIREDShare this page